Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase

Yuka Ito; Junko Okuda-Shimazaki; Wakako Tsugawa; Noya Loew; Isao Shitanda; Chi-En Lin; Jeffrey La Belle; Koji Sode

doi:10.1016/j.bios.2019.01.018

Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase

Biosens Bioelectron. 2019 Mar 15:129:189-197. doi: 10.1016/j.bios.2019.01.018. Epub 2019 Jan 16.

Authors

Yuka Ito¹, Junko Okuda-Shimazaki², Wakako Tsugawa¹, Noya Loew³, Isao Shitanda⁴, Chi-En Lin⁵, Jeffrey La Belle⁵, Koji Sode⁶

Affiliations

¹ Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
² Ultizyme International Ltd., 1-13-16, Minami, Meguro, Tokyo 152-0013, Japan; Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 and North Carolina State University, Raleigh, NC 27695, USA.
³ Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 and North Carolina State University, Raleigh, NC 27695, USA.
⁴ Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
⁵ School of Biological and Health System Engineering, Ira A. Fulton Schools of Engineering, Arizona State University, P.O.Box 879709, Tempe, AZ 85287-9719, USA.
⁶ Department of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan; Ultizyme International Ltd., 1-13-16, Minami, Meguro, Tokyo 152-0013, Japan; Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 and North Carolina State University, Raleigh, NC 27695, USA. Electronic address: ksode@email.unc.edu.

PMID: 30721794
DOI: 10.1016/j.bios.2019.01.018

Abstract

Faradaic electrochemical impedance spectroscopy (faradaic EIS) is an attractive measurement principle for biosensors. However, there have been no reports on sensors employing direct electron transfer (DET)-type redox enzymes based on faradaic EIS principle. In this study, we have attempted to construct the 3rd-generation faradaic enzyme EIS sensor, which used DET-type flavin adenine dinucleotide (FAD) dependent glucose dehydrogenase (GDH) complex, to elucidate its characteristic properties as well as to investigate its potential application as the future immunosensor platform. The gold disk electrodes (GDEs) with DET-type FADGDH prepared using self-assembled monolayer (SAM) showed the glucose concentration dependent impedance change, which was confirmed by the change in the charge transfer resistance (R_ct). The Δ(1/R_ct) values were also affected by DC bias potential and the length of SAM. Based on the Nyquist plot and Bode plot simulations, glucose sensing by imaginary impedance monitoring under fixed frequency (5 mHz) was carried out, revealing the higher sensitivity at low glucose concentration with wider linear range (0.02-0.2 mM). Considering this high sensitivity toward glucose, the 3rd-generation faradaic enzyme EIS sensor would provide alternative platform for future impedimetric immunosensing system, which does not use redox probe.

Keywords: Charge transfer resistance; Direct electron transfer; FAD dependent glucose dehydrogenase complex; Faradaic electrochemical impedance spectroscopy; Imaginary impedance monitoring; Impedimetric biosensor.

MeSH terms

Bacteria / enzymology
Biosensing Techniques / methods*
Electric Impedance
Electrodes
Electron Transport
Flavin-Adenine Dinucleotide / chemistry
Glucose / analysis*
Glucose 1-Dehydrogenase / chemistry*
Gold / chemistry

Substances

Flavin-Adenine Dinucleotide
Gold
Glucose 1-Dehydrogenase
Glucose