To effectively control malaria, it is necessary to identify landscapes that are most favorable for its transmission. To achieve this goal, the authors developed landscape malariological zoning of Southern Uzbekistan as the most problematic area for malaria in the country. For landscape malariological zoning, the investigators applied the method developed by A.Ya.Lysenko et al. (1956), which allowed identification of different types of malariogenic landscapes, by using the existing scheme of physical and geographical zoning. The existing physical and geographical areas identified were assigned malariological characteristics, which permitted assessment of the landscapes of Southern Uzbekistan from the point of view of whether there is a risk of local malaria transmission. The zoning could identify 5 types of malariogenic landscapes. The most malariologically dangerous areas are the landscapes of lowland river valleys and irrigated (irrigation channel)-lands, where there is the larg- est area of anophelogenic reservoirs. The malariological situation in the low-hill landscapes depends on the situation in the lowland river and irrigation channel landscapes. An epidemic outbreak may occur in the mid-mountain landscapes if a large number of an infection carrier and source are present. The results can be used to optimize anti-malarial interventions, prognosis, and prevention of malaria resumption in the area under study.