RNA-Seq profiling in peripheral blood mononuclear cells of amyotrophic lateral sclerosis patients and controls

Sci Data. 2019 Feb 5:6:190006. doi: 10.1038/sdata.2019.6.

Abstract

Coding and long non-coding RNA (lncRNA) metabolism is now revealing its crucial role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we present a dataset obtained via Illumina RNA-seq analysis on Peripheral Blood Mononuclear Cells (PBMCs) from sporadic and mutated ALS patients (mutations in FUS, TARDBP, SOD1 and VCP genes) and healthy controls. This dataset allows the whole-transcriptome characterization of PBMCs content, both in terms of coding and non-coding RNAs, in order to compare the disease state to the healthy controls, both for sporadic patients and for mutated patients. Our dataset is a starting point for the omni-comprehensive analysis of coding and lncRNAs, from an easy to withdraw, manage and store tissue that shows to be a suitable model for RNA profiling in ALS.

Publication types

  • Dataset
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyotrophic Lateral Sclerosis / genetics*
  • DNA-Binding Proteins / genetics
  • Gene Expression Profiling*
  • Humans
  • Leukocytes, Mononuclear / metabolism*
  • Mutation
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / physiology
  • RNA-Binding Protein FUS / genetics
  • Superoxide Dismutase-1 / genetics
  • Valosin Containing Protein / genetics

Substances

  • DNA-Binding Proteins
  • FUS protein, human
  • RNA, Long Noncoding
  • RNA-Binding Protein FUS
  • SOD1 protein, human
  • TARDBP protein, human
  • Superoxide Dismutase-1
  • VCP protein, human
  • Valosin Containing Protein