Novel miRNA expression in the delta opioid signaling pathway mediated cell survivability in an in vitro model of ER stress

M Erfath Thanjeem Begum; Himadri Shekhaar Baul; Katari Venkatesh; Dwaipayan Sen

doi:10.1016/j.nano.2019.01.009

Novel miRNA expression in the delta opioid signaling pathway mediated cell survivability in an in vitro model of ER stress

Nanomedicine. 2019 Apr:17:150-187. doi: 10.1016/j.nano.2019.01.009. Epub 2019 Feb 1.

Authors

M Erfath Thanjeem Begum¹, Himadri Shekhaar Baul¹, Katari Venkatesh¹, Dwaipayan Sen²

Affiliations

¹ Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India.
² Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India.. Electronic address: dwaipayan.sen@vit.ac.in.

PMID: 30716419
DOI: 10.1016/j.nano.2019.01.009

Abstract

Micro RNAs (miRNAs) are small non-coding RNAs which bind to the 3'-untranslated region of a mature mRNA to induce degradation; thereby regulating gene expression. It is reported that dysregulated miRNAs involved in neurodegenerative diseases including Parkinson's disease, could play a significant role as prognostic markers and therapeutic targets. Neuroprotective effect of delta opioid receptors (DOR) and its known miRNA regulation against endoplasmic reticulum (ER) stress have been reported previously by our lab. Current study focuses on understanding the regulation of novel miRNAs by DOR under ER stress. Novel miRNAs were identified for three different samples; control, tunicamycin (ER stress inducer), and tunicamycin+DADLE (DOR agonist). Differentially regulated miRNAs between the different samples were identified and pathway/target genes analysis was carried out. The results suggest that following DOR activation novel miRNAs like xxx-m0073-3p, xxx-m0225-3p, xxx-m0088-3p, xxx-m0098-5p etc. could regulate cell survival mechanisms in neuronal cells (SH-SY5Y) under ER stress.

Keywords: Cell survivability; DOR activation; ER stress; Novel miRNAs; Parkinson's disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Endoplasmic Reticulum Stress*
Gene Expression Profiling
Humans
MicroRNAs / genetics*
Parkinson Disease / genetics
Parkinson Disease / metabolism
Receptors, Opioid, delta / genetics*
Receptors, Opioid, delta / metabolism
Signal Transduction

Substances

MicroRNAs
Receptors, Opioid, delta