How to create a portable and quick way to detect multiple coexisting toxins is closely related to everyone's health. In this paper, we have established a real-time mycotoxin detection system that combined shape-encoded hydrogel particle preparation technology and image processing technology with smartphone portable devices. First, hydrogel microparticles containing a specific recognition toxin aptamer were programmable synthesized by stop-flow lithography. The hydrogel particles prepared by us had clear, variable signals and high coding capacity. Then, the indirect competitive detection based on aptamers was simple and rapid; the total reaction time was no more than 1 h 45 min and the image processing process was no more than 10 s. Finally, images could be captured by cameras on portable devices and smartphones. The self-built Android app that used the image recognition program installed on the smartphone would analyze the image and return the results in real time. The results showed that the detection limit reached 0.1 ng/mL, which was lower than the standard. In summary, this platform provides a fast, portable, high-throughput detection solution for real-time detection of mycotoxins, with excellent application prospects.
Keywords: POCT device; hydrogel; image recognition; mycotoxins; stop-flow lithography.