HPLC-ESI-MSn Identification and NMR Characterization of Glucosyloxybenzyl 2 R-Benzylmalate Deriva-Tives from Arundina Graminifolia and Their Anti-Liver Fibrotic Effects In Vitro

Molecules. 2019 Jan 31;24(3):525. doi: 10.3390/molecules24030525.

Abstract

Four new glucosyloxybenzyl 2R-benzylmalate derivatives, named Arundinoside H (2), I (5), J (6), K (8) as well as four known compounds Arundinoside D (1), G (3), F (4), E (7) were isolated and characterized by a combination of chemical and spectroscopic methods, including HR-ESI-MS, 1D and 2D NMR experiments. Besides, 24 unreported compounds were inferred from ESI-MSn data. The anti-liver fibrotic activities of the isolates were determined as proliferation inhibition of lipopolysaccharide (LPS)-induced activation of rat hepatic stellate cells (HSC-T6). The result suggested Arundinosides D, H, F, I and K showed moderate inhibitory effects in vitro.

Keywords: Arundina graminifolia; MSn fragmentation pattern; anti-liver fibrotic effects; glucosyloxybenzyl 2R-benzylmalates.

MeSH terms

  • Animals
  • Cell Line
  • Chromatography, High Pressure Liquid*
  • Disease Models, Animal
  • Hepatocytes / drug effects
  • Hepatocytes / metabolism
  • Liver Cirrhosis / drug therapy
  • Liver Cirrhosis / etiology
  • Liver Cirrhosis / pathology
  • Magnetic Resonance Spectroscopy*
  • Mandelic Acids / chemistry*
  • Mandelic Acids / pharmacology*
  • Molecular Structure
  • Orchidaceae / chemistry*
  • Spectrometry, Mass, Electrospray Ionization*

Substances

  • Mandelic Acids
  • benzylmandelate