A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp. spectabilis: Extraction, purification and structural features

Masoumeh Beigi; Kambiz Jahanbin

doi:10.1016/j.ijbiomac.2019.01.178

A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp. spectabilis: Extraction, purification and structural features

Int J Biol Macromol. 2019 May 1:128:648-654. doi: 10.1016/j.ijbiomac.2019.01.178. Epub 2019 Jan 30.

Authors

Masoumeh Beigi¹, Kambiz Jahanbin²

Affiliations

¹ Shahrood University of Technology, Faculty of Agriculture, Department of Food Science and Technology, Shahrood, Iran.
² Shahrood University of Technology, Faculty of Agriculture, Department of Food Science and Technology, Shahrood, Iran. Electronic address: Jahanbin@shahroodut.ac.ir.

PMID: 30708016
DOI: 10.1016/j.ijbiomac.2019.01.178

Abstract

A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100. Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively. Monosaccharide composition analysis showed that ESP consisted of glucose and mannose at a molar ratio of 1.0:2.9. Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR). The repeating unit of ESP was determined as following.

Keywords: Eremurus spectabilis; Glucomannan; NMR spectroscopy; Structural analysis.

MeSH terms

Asparagales / chemistry*
Carbohydrate Sequence
Methylation
Monosaccharides / analysis
Plant Roots / chemistry*
Polysaccharides / chemistry*
Polysaccharides / isolation & purification*
Polysaccharides / metabolism
Solubility
Water / chemistry*

Substances

Monosaccharides
Polysaccharides
Water