Simultaneous colorimetric determination of acute myocardial infarction biomarkers by integrating self-assembled 3D gold nanovesicles into a multiple immunosorbent assay

Qinli Pu; Xuanhua Yang; Yongcan Guo; Tao Dai; Tingyan Yang; Xinying Ou; Junlong Li; Shangchun Sheng; Guoming Xie

doi:10.1007/s00604-019-3242-y

Simultaneous colorimetric determination of acute myocardial infarction biomarkers by integrating self-assembled 3D gold nanovesicles into a multiple immunosorbent assay

Mikrochim Acta. 2019 Feb 1;186(3):138. doi: 10.1007/s00604-019-3242-y.

Authors

Qinli Pu¹, Xuanhua Yang², Yongcan Guo³, Tao Dai¹, Tingyan Yang¹, Xinying Ou¹, Junlong Li⁴, Shangchun Sheng⁵, Guoming Xie⁶

Affiliations

¹ Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
² Department of Gastrointestinal Surgery, Second Clinical Medical Institution of North Sichuan Medical College, Nanchong Central Hospital, Nanchong, Sichuan, 637000, People's Republic of China.
³ Clinical Laboratory of Traditional Chinese Medicine Hospital Affiliated to Southwest Medical University, Luzhou, Sichuan, 646000, People's Republic of China.
⁴ University-Town Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
⁵ Clinical Laboratory of Hospital Affiliated to Chengdu University, Sichuan, 610081, People's Republic of China.
⁶ Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China. guomingxie@cqmu.edu.cn.

PMID: 30707310
DOI: 10.1007/s00604-019-3242-y

Abstract

An improved enzyme-free immunosorbent assay is described for the simultaneous detection of the myocardial infarction biomarkers N-terminal pro B type natriuretic peptide (NT-proBNP), creatine kinase-MB (CK-MB), and cardiac muscle troponin T (cTnT). The assay integrates 3D gold nanovesicles (GNVs) and three allochroic agents (phenolphthalein, methyl red, bromothymol blue). The pH regulated allochroic agents were enwrapped in GNVs to acts as ultrasensitive nanoprobes. Loading can be controlled by adjusting the temperature to efficiently load and release the allochroic agents. This bare-eye multicolor assay has limits of detection of 70 pg·mL^-1 for NT-proBNP, 910 pg·mL^-1 for CK-MB, and 7.8 pg·mL^-1 for cTnT. Other features include (a) a linear range that extends over a wide range and sometimes is better than conventional HRP-based immunoassays, and (b) a precision that is comparable to immunofluorescence assays as used in the clinical laboratory. Graphical abstract Schematic presentation of an improved enzyme-free immunosorbent assay (EFISA). It integrates 3D gold nano-vesicles (GNVs) and allochroic agents for the simultaneous detection of acute myocardial infarction (AMI) biomarkers (N-terminal prohormone of brain natriuretic peptide (NT-proBNP), kinase-muscle/brain test (CK-MB), and cardiac muscle troponin (cTnT)).

Keywords: Allochroic agents; Cardiac muscle troponin T; Creatine kinase-MB; Enzyme-free immunosorbent assay; Gold nanovesicles; Multiplex detection; N-terminal pro B type natriuretic peptide; Nanoprobes; Plasma samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers / blood*
Biosensing Techniques
Colorimetry
Coloring Agents / chemistry
Creatine Kinase, MB Form / blood
Gold / chemistry*
Humans
Hydrogen-Ion Concentration
Immunosorbents / chemistry*
Limit of Detection
Metal Nanoparticles / chemistry*
Myocardial Infarction / diagnosis*
Myocardium / metabolism
Natriuretic Peptide, Brain / blood
Particle Size
Peptide Fragments / blood
Surface Properties
Temperature
Troponin T / blood

Substances

Biomarkers
Coloring Agents
Immunosorbents
Peptide Fragments
Troponin T
pro-brain natriuretic peptide (1-76)
Natriuretic Peptide, Brain
Gold
Creatine Kinase, MB Form