Improving nuclear morphometry imaging with real-time and low-cost line-scanning confocal microendoscope

Opt Lett. 2019 Feb 1;44(3):654-657. doi: 10.1364/OL.44.000654.

Abstract

Fiber-optic endomicroscopy is a minimally invasive tool to probe disease progression with subcellular resolution. In this Letter, we demonstrate a low-cost and compact fluorescence microendoscope capable of line-scanning confocal imaging by synchronizing a digital light projector with a CMOS camera. We present the digital aperture design to enable real-time confocal imaging, and we implement parallel illumination to improve the optical sectioning performance. Furthermore, we show that the confocal microendoscope can enhance visualization of disease-associated features when imaging highly scattering esophageal specimens.

MeSH terms

  • Costs and Cost Analysis*
  • Endoscopes / economics*
  • Esophagus / diagnostic imaging
  • Humans
  • Microscopy, Confocal / economics*
  • Microscopy, Confocal / instrumentation*
  • Optical Fibers
  • Time Factors