Chitosan capped copper oxide/copper nanoparticles encapsulated microbial resistant nanocomposite films

Tippabattini Jayaramudu; Kokkarachedu Varaprasad; Radha D Pyarasani; K Koteshwara Reddy; Kanderi Dileep Kumar; A Akbari-Fakhrabadi; R V Mangalaraja; John Amalraj

doi:10.1016/j.ijbiomac.2019.01.145

Chitosan capped copper oxide/copper nanoparticles encapsulated microbial resistant nanocomposite films

Int J Biol Macromol. 2019 May 1:128:499-508. doi: 10.1016/j.ijbiomac.2019.01.145. Epub 2019 Jan 27.

Authors

Tippabattini Jayaramudu¹, Kokkarachedu Varaprasad², Radha D Pyarasani³, K Koteshwara Reddy⁴, Kanderi Dileep Kumar⁵, A Akbari-Fakhrabadi⁶, R V Mangalaraja⁷, John Amalraj⁸

Affiliations

¹ Laboratory of Materials Science, Instituto de Quimica de Recursos Naturales, Universidad de Talca, 747 Talca, Chile. Electronic address: jtibbabattini@utalca.cl.
² Centre de Investigacion de Polimeros Avanzados, CIPA, avenida Collao 1202, Edificio de Laoratorios, Concepcion, Chile.
³ Vicerrectoria de Investigacion y Postgrado, Universidad Catolica del Maule, 3460000 Talca, Chile.
⁴ Laboratory of Materials Science, Instituto de Quimica de Recursos Naturales, Universidad de Talca, 747 Talca, Chile.
⁵ Dept. of Microbiology, Sri Krishnadevaraya University, Ananthapuramu 515003, Andhra Pradesh, India.
⁶ Advanced Materials Laboratory, Department of Mechanical Engineering, University of Chile, Beauchef, 851 Santiago, Chile.
⁷ Advanced Ceramics and Nanotechnology Laboratory, Department of Materials Engineering, Faculty of Engineering, University of Concepcion, Concepcion 407-0409, Chile.
⁸ Laboratory of Materials Science, Instituto de Quimica de Recursos Naturales, Universidad de Talca, 747 Talca, Chile. Electronic address: jamalraj@utalca.cl.

PMID: 30699337
DOI: 10.1016/j.ijbiomac.2019.01.145

Abstract

Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field. This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs). Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu²⁺ ions using sodium hydroxide and ascorbic acid. TEM analysis revealed the morphology as rod-type nanoflakes for CHCuO and a spherical shape for CHCu NPs with ~7 ± 2 nm size. Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria. The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials. The results suggested that the synthesized materials can be used in wound dressing applications.

Keywords: CHCu NPs; CHCuO NPs; Chitosan.

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Bacillus / drug effects
Capsules
Chitosan / chemistry*
Copper / chemistry*
Copper / pharmacology*
Drug Resistance, Bacterial / drug effects*
Escherichia coli / drug effects
Metal Nanoparticles / chemistry*
Nanocomposites / chemistry*
Surface Properties
Temperature

Substances

Anti-Bacterial Agents
Capsules
Copper
Chitosan
cupric oxide