EGTA (ethylene glycol bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid) and BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid) are well-known Ca2+ chelators that have four carboxylates, two nitrogen atoms and two ether oxygen atoms. In the present study, we prepared EGTQ (N,N,N',N'-tetrakis(2-quinolylmethyl)-1,2-bis(2-aminoethoxy)ethane) and BAPTQ (N,N,N',N'-tetrakis(2-quinolylmethyl)-1,2-bis(2-aminophenoxy)ethane) as quinoline alternatives of EGTA and BAPTA, respectively. In methanol-HEPES buffer solution (9 : 1, 50 mM HEPES, 0.1 M KCl, pH = 7.5), EGTQ exhibits fluorescence enhancement induced by Zn2+ and Cd2+ with poor selectivity, but BAPTQ did not exhibit a fluorescence response to either metal ion. Introduction of three methoxy substituents at the 5,6,7-positions of each quinoline moiety in BAPTQ specifically enhanced the fluorescence intensity of the Cd2+ complex, establishing the Cd2+-specific probe TriMeOBAPTQ (N,N,N',N'-tetrakis(5,6,7-trimethoxy-2-quinolylmethyl)-1,2-bis(2-aminophenoxy)ethane). In contrast, TriMeOEGTQ (N,N,N',N'-tetrakis(5,6,7-trimethoxy-2-quinolylmethyl)-1,2-bis(2-aminoethoxy)ethane) maintains a poor Cd2+/Zn2+ selectivity in its fluorescence response. Although the crystal structures of Cd2+/Zn2+ complexes with EGTQ and BAPTQ derivatives reveal the formation of multiple components including mononuclear and dinuclear complexes, the dinuclear Cd2+ and Zn2+ complexes with a linearly extended structure are regarded as possible fluorescent species in the solution. The conformational restriction of BAPTQ due to the orthophenylene moieties in the molecular skeleton is responsible for the formation of the weakly fluorescent, OH-bridged dizinc complex, which is critical to the strict Cd2+-specificity in the fluorescence response of TriMeOBAPTQ.