The aim of this study was to investigate and analyze the results of transthoracic needle biopsy of lung tumors under CT navigation. There were carried out more than 100 transthoracic needle biopsies of lung tumors. The analysis of immediate results of the applicability of this technique was performed. The average period of hospitalization after the puncture was 1.4 days (1-7). A number of pneumothorax that required drain- ing the pleural cavity was 8. The median of duration of drain- ing the pleural cavity was 6 of 8 patients (5-6.5) days. More severe complications and deaths were not. The histological conclusion was obtained in 100% of cases, in 72 patients the diagnosis of a malignant lung tumor was firstly verified, 12 cases showed a false-negative result. Diagnosis of lung cancer was excluded in 16 patients. The sensitivity of the method was 86%, specificity - 100% and accuracy - 88%. Therefore transthoracic needle biopsy of lung tumors with minimal risk to the patient allows receiving the morphological verification of peripheral lung tumors.