[COMPARISON OF DIAGNOSTIC EFFECTIVENESS OF METHODS OF DETECTION OF COXIELLA BURNETII IN BLOOD OF PATIENTS WITH Q FEVER BASED ON AMPLIFI- CATION OF 16S rRNA GENE FRAGMENTS (STANDARD PCR) AND groEL GENE (REAL- TIME PCR)]

Zh Mikrobiol Epidemiol Immunobiol. 2016 May:(3):70-74.
[Article in Russian]

Abstract

Aim: Comparison of diagnostic capabilities of 2 variants of PCR for detection of Coxiella burneli persistence in dynamics of infectious process in patients with Q fever.

Materials and methods: 110 samples of clinical material, obtained from patients with Q fever in an endemic region for this infection (Astrakhan region), were studied. The samples were studied in a standard PCR (marker - 16S rRNA gene fragment) and in real-time. PCR (RT-PCR) (marker - groEL gene fragment).

Results: Both markers were established to be perspective for detection of C. burnetii DNA in clinical material, and RT-PCR detects positive result including late stages of the disease (illness day 21 - 31).

Conclusion: This study is the first Russian publication on comparison on different PCR variants for detection of C. burnetii in blood of Q fever patients in dynamics of the infectious process.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Bacterial Proteins / genetics*
  • Chaperonin 60 / genetics*
  • Coxiella burnetii / genetics*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Q Fever* / diagnosis
  • Q Fever* / genetics
  • RNA, Bacterial / genetics*
  • RNA, Ribosomal, 16S / genetics*
  • Real-Time Polymerase Chain Reaction*

Substances

  • Bacterial Proteins
  • Chaperonin 60
  • RNA, Bacterial
  • RNA, Ribosomal, 16S