Targeted Metagenomics of Microbial Diversity in Free-Living Amoebae and Water Samples

Methods Mol Biol. 2019:1921:421-428. doi: 10.1007/978-1-4939-9048-1_26.

Abstract

The presence of Legionella spp. in natural and man-made water systems is a great public health concern and heavily depends on the presence of free-living amoebae. Taking advantage of the development and affordability of next-generation sequencing technology, we present here a method to characterize the whole bacterial community directly from water samples, as well as from isolated free-living amoebae.

Keywords: 16S rRNA; Bacteria; Free-living amoeba; High-throughput sequencing; Targeted metagenomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amoeba / microbiology*
  • Biodiversity
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Metagenome*
  • Metagenomics* / methods
  • Microbiota*
  • RNA, Ribosomal, 16S
  • Water Microbiology*

Substances

  • RNA, Ribosomal, 16S