The study of Legionella pneumophila interactions with host mitochondria during infection has been historically limited by the techniques available to analyze and quantify mitochondrial dynamics and activity in living cells. Recently, new, powerful techniques such as high-content microscopy or mitochondrial respiration assays (Seahorse) have been developed to quantitatively analyze mitochondrial parameters. Here we present state-of-the-art methods adapted to analyze mitochondrial dynamics and activity during Legionella infection of living human primary macrophages.
Keywords: Bioenergetics; High-content microscopy; Legionella pneumophila; Living-cell assays; MitoTracker dyes; Mitochondrial dynamics; Mitochondrial fission; Mitochondrial morphology; Mitochondrial respiration assay; Seahorse.