Mig-14 may contribute to Salmonella enterica serovar Typhi resistance to polymyxin B by decreasing the permeability of the outer-membrane and promoting the formation of biofilm

Xiumei Sheng; Weiwei Wang; Long Chen; Hong Zhang; Ying Zhang; Shungao Xu; Huaxi Xu; Xinxiang Huang

doi:10.1016/j.ijmm.2019.01.001

Mig-14 may contribute to Salmonella enterica serovar Typhi resistance to polymyxin B by decreasing the permeability of the outer-membrane and promoting the formation of biofilm

Int J Med Microbiol. 2019 Mar;309(2):143-150. doi: 10.1016/j.ijmm.2019.01.001. Epub 2019 Jan 16.

Authors

Xiumei Sheng¹, Weiwei Wang², Long Chen², Hong Zhang², Ying Zhang², Shungao Xu², Huaxi Xu², Xinxiang Huang³

Affiliations

¹ Department of Central lab, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, 212002, China; Department of Biochemistry and Molecular Biology, Jiangsu University School of Medicine, Zhenjiang, Jiangsu, 212013, China.
² Department of Biochemistry and Molecular Biology, Jiangsu University School of Medicine, Zhenjiang, Jiangsu, 212013, China.
³ Department of Biochemistry and Molecular Biology, Jiangsu University School of Medicine, Zhenjiang, Jiangsu, 212013, China. Electronic address: huxinx@ujs.edu.cn.

PMID: 30685290
DOI: 10.1016/j.ijmm.2019.01.001

Abstract

Mig-14 is essential for Salmonella enterica serovar Typhimurium (S. Typhimurium) resistance to antimicrobial peptides, including polymyxin B (PB). However, the molecular mechanism is as yet unknown. In this study, we demonstrated that mig-14 also played a crucial role in Salmonella enterica serovar Typhi (S. Typhi) resistance to PB. A series of genes associated with drug-resistance controlled by Mig-14 were identified in the presence of PB. Among which, ompF and ompC were up-regulated 8 and 6 folds in mig-14 mutant (Δmig-14) strains, respectively. Further, the deletion of ompF or/and ompC in Δmig-14 strains decreased their sensitivity to PB. Besides, the biofilm formation ability was reduced in Δmig-14 strains. Our results indicate that Mig-14 may contribute to PB resistance in S. Typhi by decreasing the permeability of the outer membrane and promoting biofilm formation.

Keywords: Antimicrobial resistance; Biofilm; S. Typhi; mig-14; ompC; ompF.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Outer Membrane Proteins / genetics
Bacterial Outer Membrane Proteins / metabolism*
Biofilms / growth & development*
Cell Membrane / drug effects*
Drug Resistance, Bacterial*
Gene Deletion
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Permeability
Polymyxin B / pharmacology*
Salmonella typhi / drug effects*
Salmonella typhi / genetics
Salmonella typhi / growth & development

Substances

Anti-Bacterial Agents
Bacterial Outer Membrane Proteins
Polymyxin B