A single injection of corifollitropin alfa supplemented with human chorionic gonadotropin increases follicular recruitment and transferable embryos in the rabbit

Reprod Domest Anim. 2019 Apr;54(4):696-701. doi: 10.1111/rda.13411. Epub 2019 Feb 18.

Abstract

Superovulation protocols are designed to achieve maximum embryo yields. Nevertheless, ovarian response control and the quality of obtained embryos are still a challenge. On the other hand, to save the superovulated embryos until their subsequent use, it is usual to cryopreserve them, so it is also crucial to assess their cryotolerance. The aim of this study was to compare the efficacy of a single injection of corifollitropin alfa (FSH-CTP) alone or supplemented with human chorionic gonadotropin (hCG) and to determine the impact of this stimulation on in vitro and in vivo development of fresh or devitrified embryos. Our outcomes showed that ovulation rate and recovered embryos were significantly increased when hCG was used. In vitro development of fresh and devitrified embryos and survival at birth were not significantly affected by superstimulation treatment. Results of this study suggest that a single injection of long-acting FSH-CTP supplemented with hCG can be effectively used in rabbits to elicit an increase in ovulation rate and number of recovered embryos. Furthermore, we demonstrated that hCG supplementation had no negative effects in embryo cryosurvival and development, showing similar survival rate at birth than FSH-CTP alone group.

Keywords: embryo viability; hCG; long-acting FSH; rabbit; superovulation; vitrification.

Publication types

  • Clinical Trial, Veterinary

MeSH terms

  • Animals
  • Chorionic Gonadotropin / administration & dosage
  • Chorionic Gonadotropin / pharmacology
  • Cryopreservation / veterinary
  • Drug Therapy, Combination
  • Embryo Transfer / veterinary*
  • Female
  • Follicle Stimulating Hormone, Human / administration & dosage
  • Follicle Stimulating Hormone, Human / pharmacology*
  • Insemination, Artificial / veterinary
  • Ovarian Follicle
  • Rabbits / embryology
  • Rabbits / physiology*
  • Random Allocation
  • Superovulation / drug effects
  • Vitrification

Substances

  • Chorionic Gonadotropin
  • Follicle Stimulating Hormone, Human
  • follicle stimulating hormone, human, with HCG C-terminal peptide