[The development of Endo growth medium for detection and identification of enterobacteria]

Klin Lab Diagn. 2018;63(2):110-113. doi: 10.18821/0869-2084-2018-63-2-110-113.
[Article in Russian]

Abstract

The purpose of study is to develop a traditional Endo's growth medium with the view of suppression of swarming of Proteus vulgaris and Proteus mirabilis. The object of study was a commercial dry Endo's growth medium. The inoculation of reference strains of enterobacteria and clinical sample (feces, urine, phlegm) was implemented according the normative documents. It is demonstrated that brining into Endo's growth medium tryptophan and sodium salts of bile acids in a particular ratio of their concentrations suppressed swarming of Proteus vulgaris and Proteus mirabilis. The obtained results demonstrated that application of enhanced Endo's growth medium significantly increases its differential and diagnostic characteristics.

Keywords: Endo's growth medium; enterobacteria; odium salts of bile acids; phenomenon of "swarming" of P. vulgaris and P. mirabilis; tryptophan.

MeSH terms

  • Culture Media
  • Proteus mirabilis*
  • Proteus vulgaris*

Substances

  • Culture Media