Molecular cloning of IL-6, IL-10, IL-11, IFN-ɤ and modulation of pro- and anti-inflammatory cytokines in cobia (Rachycentron canadum) after Photobacterium damselae subsp. piscicida infection

Hung B Tran; Shih-Chu Chen; Hso-Chi Chaung; Ta-Chih Cheng

doi:10.1016/j.cbpb.2019.01.004

Molecular cloning of IL-6, IL-10, IL-11, IFN-ɤ and modulation of pro- and anti-inflammatory cytokines in cobia (Rachycentron canadum) after Photobacterium damselae subsp. piscicida infection

Comp Biochem Physiol B Biochem Mol Biol. 2019 Apr:230:10-18. doi: 10.1016/j.cbpb.2019.01.004. Epub 2019 Jan 18.

Authors

Hung B Tran¹, Shih-Chu Chen², Hso-Chi Chaung², Ta-Chih Cheng³

Affiliations

¹ Laboratory of Molecular Fish Immunology and Genetics, Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology,Pingtung 91201, Taiwan; Research Center for Animal Biologics, National Pingtung University of Science and Technology,Pingtung 91201, Taiwan.
² Research Center for Animal Biologics, National Pingtung University of Science and Technology,Pingtung 91201, Taiwan; Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, No. 1, Shuefu Road, Neipu, Pingtung 91201, Taiwan.
³ Laboratory of Molecular Fish Immunology and Genetics, Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology,Pingtung 91201, Taiwan; Research Center for Animal Biologics, National Pingtung University of Science and Technology,Pingtung 91201, Taiwan. Electronic address: cheng.tachih@gmail.com.

PMID: 30665028
DOI: 10.1016/j.cbpb.2019.01.004

Abstract

Photobacterium damselae subsp. piscicida (P. damselae subsp. piscicida) is the agent of Photobacteriosis, a serious fish disease that produces an acute infection and high mortality in farmed cobia. It has been proved that regulation of pro- and anti-inflammatory cytokines play a central role in initiation of proper inflammatory responses against bacterial infection. Here we have analyzed the expression of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, IL-8 and IFN-ɤ) and anti-inflammatory cytokines (IL-10 and IL-11) in spleen and head kidney during acute P. damselae subsp. piscicida infection of cobia. Our data revealed that cytokines tested showed distinct patterns of expression. While TNF-α and IL-8 showed a decay pattern of expression, IL-1β response was quite late. Moreover, P. damselae subsp. piscicida infection induced the simultaneous expressions of pro-inflammatory (IL-6, IFN-ɤ) and anti-inflammatory (IL-10, IL-11) cytokines. Together these results indicate the innate immunity of cobia is actively suppressed by P. damselae subsp. piscicida.

Keywords: Anti-inflammatory cytokines; Photobacterium damselae subsp. piscicida; Pro-inflammatory cytokines; Rachycentron canadum.

MeSH terms

Animals
Cytokines / metabolism*
Fish Diseases / immunology
Fish Diseases / metabolism*
Fish Diseases / microbiology
Fish Proteins / metabolism*
Gram-Negative Bacterial Infections / immunology
Gram-Negative Bacterial Infections / metabolism*
Gram-Negative Bacterial Infections / microbiology
Inflammation Mediators / metabolism*
Interferon-gamma / metabolism
Interleukin-10 / metabolism
Interleukin-11 / metabolism
Interleukin-6 / metabolism
Perciformes / metabolism*
Perciformes / microbiology
Photobacterium / physiology*

Substances

Cytokines
Fish Proteins
Inflammation Mediators
Interleukin-11
Interleukin-6
Interleukin-10
Interferon-gamma