Intrinsic peroxidase-like activity of Cu2ZnSn(SxSe1-x)4 nanocrystals, and their application to the colorimetric detection of H2O2

Peng Ju; Jinfeng Ding; Bing Wang; Wen Li; Fenghua Jiang; Xiuxun Han; Chengjun Sun; Chi Wu

doi:10.1007/s00604-018-3185-8

Intrinsic peroxidase-like activity of Cu₂ZnSn(S_xSe_1-x)₄ nanocrystals, and their application to the colorimetric detection of H₂O₂

Mikrochim Acta. 2019 Jan 19;186(2):118. doi: 10.1007/s00604-018-3185-8.

Authors

Peng Ju^{1

2}, Jinfeng Ding², Bing Wang², Wen Li³, Fenghua Jiang², Xiuxun Han⁴, Chengjun Sun^{5

6}, Chi Wu⁷

Affiliations

¹ Institute of Marine Science and Technology, Shandong University, 72 Binhai Road, Qingdao, 266237, People's Republic of China.
² Key Laboratory of Marine Bioactive Substances and Analytical Technology, Marine Ecology Center, The First Institute of Oceanography, State Oceanic Administration (SOA), 6 Xianxialing Road, Qingdao, 266061, People's Republic of China.
³ Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, 111 the Northbound 1 of the Second Ring Road, Chengdu, 610031, China.
⁴ Institute of Semiconductor Materials, Jiangxi University of Science and Technology, 86 Hongqi Road, Ganzhou, 341000, People's Republic of China.
⁵ Key Laboratory of Marine Bioactive Substances and Analytical Technology, Marine Ecology Center, The First Institute of Oceanography, State Oceanic Administration (SOA), 6 Xianxialing Road, Qingdao, 266061, People's Republic of China. csun@fio.org.cn.
⁶ Laboratory of Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), 1 Wenhai Road, Qingdao, 266237, People's Republic of China. csun@fio.org.cn.
⁷ Institute of Marine Science and Technology, Shandong University, 72 Binhai Road, Qingdao, 266237, People's Republic of China. qwcnsd@163.com.

PMID: 30661119
DOI: 10.1007/s00604-018-3185-8

Abstract

Nanocrystals (NCs) of type Cu₂ZnSn(S_xSe_1-x)₄ (CZTSSe) were prepared via a solvothermal approach. They are shown to be highly efficient peroxidase (POx) mimics for colorimetric detection of H₂O₂. By varying the molar ratio of S and Se during preparation, the NCs showed different crystal structures, morphologies, surface properties, and POx-like activities. Among them, the type CZTSSe-0.25 NCs exhibit the strongest POx-like activities towards the catalytic oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of H₂O₂ to generate a blue product. The enhanced activity is attributed to its more negative potential and larger specific surface of the NCs. Based on these findings, a rapid and ultrasensitive method was developed for the visual and colorimetric determination of H₂O₂. The method is selective, and the NCs are reusable and long-term stable. The detection limit of H₂O₂ is 50 nM. Kinetic and active species trapping experiments were performed to elucidate the POx-like mechanism of the NCs. Graphical abstract Schematic presentation of the process of Cu₂ZnSn(S_xSe_1-x)₄ nanocrystals catalyzing the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ to induce a typical blue color reaction, which can be applied in colorimetric detection of H₂O₂.

Keywords: Catalytic activity; Hydrogen peroxide; Kinetics; Nanomaterials; Optical determination; Peroxidase mimetic; Sensor; TEM; TMB; XRD.

Publication types

Research Support, Non-U.S. Gov't

Abstract

Publication types

Grants and funding