Comprehensive characterization of bioactive phenols from new Brazilian superfruits by LC-ESI-QTOF-MS, and their ROS and RNS scavenging effects and anti-inflammatory activity

Food Chem. 2019 May 30:281:178-188. doi: 10.1016/j.foodchem.2018.12.106. Epub 2019 Jan 3.

Abstract

Brazilian native fruits (BNF) remain unexplored and underutilized resources with a high potential to improve human health and wellness. In our study, five new BNF (Eugenia stipitata, Sageretia elegans, Byrsonima arthropoda, Spondias mombin andRubus rosaefolius)were evaluated for their phenolic composition by LC-ESI-QTOF-MS; and for their ROS and RNS scavenging effects (ROO, O2-, NO, HOCl); in vivo anti-inflammatory activity (neutrophil migration); and in vivo acute toxicity in Galleria mellonella. Eighty-six phenolic compounds were identified, including hydroxybenzoic acids, hydroxycinnamic acids, flavonoids, anthocyanins and ellagitannins, several of which had never been reported in BNF. The BNF exhibited high antioxidant effects against biologically relevant radicals, and treated animals showed decreased neutrophil influx and NF-kB activation. Thus, these BNF are good sources of antioxidant and anti-inflammatory molecules that can be beneficial for human health as functional foods. Based on their bioactivity, they can be considered as new Brazilian superfruits.

Keywords: Anti-inflammatory activity; Antioxidant activity; Apigenin-7-O-glucoside (PubChem CID: 45933926); Coumaric acid (PubChem CID: 637542); Cyanidin-3-O-glucoside (PubChem CID: 12303203); Delphinidin 3-O-glucoside (PubChem CID: 443650); Ellagic acid (PubChem CID:5281855); Gallocatechin-gallate (PubChem CID:199472); Lambertianin C (PubChem CID: 101196665).; Naringin hydrate (PubChem CID: 6419948); Native fruits; Phenolic compounds; Quercetin-7-O-glucoside (PubChem CID: 5381351); ROS and RNS; Rutin hydrated (PubChem CID: 45479757).

MeSH terms

  • Animals
  • Anthocyanins / analysis
  • Anthocyanins / pharmacology
  • Anti-Inflammatory Agents / analysis
  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants / analysis
  • Antioxidants / pharmacology
  • Cell Movement / drug effects
  • Cell Survival / drug effects
  • Chromatography, Liquid
  • Coumaric Acids / analysis
  • Coumaric Acids / pharmacology
  • Flavonoids / analysis
  • Flavonoids / pharmacology
  • Fruit / chemistry*
  • Functional Food
  • Hydrolyzable Tannins / analysis
  • Hydrolyzable Tannins / pharmacology
  • Hydroxybenzoates / analysis
  • Hydroxybenzoates / pharmacology
  • Lepidoptera / drug effects
  • Lepidoptera / metabolism
  • Mass Spectrometry
  • Mice
  • NF-kappa B / genetics
  • NF-kappa B / metabolism
  • Neutrophils / drug effects
  • Neutrophils / metabolism
  • Phenols / analysis
  • Phenols / pharmacology*
  • Plant Extracts / analysis
  • Plant Extracts / pharmacology
  • RAW 264.7 Cells
  • Reactive Nitrogen Species / metabolism*
  • Reactive Oxygen Species / metabolism*
  • Toxicity Tests, Acute

Substances

  • Anthocyanins
  • Anti-Inflammatory Agents
  • Antioxidants
  • Coumaric Acids
  • Flavonoids
  • Hydrolyzable Tannins
  • Hydroxybenzoates
  • NF-kappa B
  • Phenols
  • Plant Extracts
  • Reactive Nitrogen Species
  • Reactive Oxygen Species
  • phenolic acid