In Vitro Suppression of CD4+ T-Cell Responses by Murine and Human Myeloid-Derived Suppressor Cells

Katerina Vlachou; Panayotis Verginis

doi:10.1007/978-1-4939-8938-6_9

In Vitro Suppression of CD4⁺ T-Cell Responses by Murine and Human Myeloid-Derived Suppressor Cells

Methods Mol Biol. 2019:1899:119-128. doi: 10.1007/978-1-4939-8938-6_9.

Authors

Katerina Vlachou¹, Panayotis Verginis²

Affiliations

¹ Laboratory of Autoimmunity and Inflammation, Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, Greece.
² Laboratory of Cellular Immunology and Immune Tolerance, Division of Immunology and Transplantation, Biomedical Research Foundation Academy of Athens, Athens, Greece. pverginis@bioacademy.gr.

PMID: 30649769
DOI: 10.1007/978-1-4939-8938-6_9

Abstract

Myeloid-derived suppressor cells (MDSCs) are myeloid precursors of macrophages, dendritic cells, and neutrophils with a prominent role in the regulation of immune responses in cancer, infection, and autoimmunity. Herein, we describe a protocol for the isolation of murine and human MDSCs and the assessment of their ability to suppress CD4⁺ T-cell responses in vitro.

Keywords: CD4+ T-cell isolation; Carboxyfluorescein succinimidyl ester (CFSE); Cell sorting; In vitro proliferation assay; Myeloid-derived suppressor cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CD4-Positive T-Lymphocytes / cytology*
Cell Proliferation
Cell Separation / methods*
Humans
Lymphocyte Activation / immunology*
Macrophages / immunology
Mice
Myeloid-Derived Suppressor Cells / cytology*