Purification and characterization of lipase by Bacillus methylotrophicus PS3 under submerged fermentation and its application in detergent industry

Pushpinder Sharma; Nivedita Sharma; Shruti Pathania; Shweta Handa

doi:10.1016/j.jgeb.2017.06.007

Purification and characterization of lipase by Bacillus methylotrophicus PS3 under submerged fermentation and its application in detergent industry

J Genet Eng Biotechnol. 2017 Dec;15(2):369-377. doi: 10.1016/j.jgeb.2017.06.007. Epub 2017 Jun 28.

Authors

Pushpinder Sharma¹, Nivedita Sharma¹, Shruti Pathania¹, Shweta Handa¹

Affiliation

¹ University of Horticulture and Forestry, Nauni, Solan, HP 173230, India.

Abstract

Lipase production bacterial isolate was isolated from soil of service station and identified as Bacillus methylotrophicus PS3 by 16SrRNA with accession number |LN999829.1|. Lipase enzyme was purified by sequential methods of ammonium sulfate precipitation and Sephadex G-100 gel column chromatography. The molecular weight of purified enzyme was 31.40 kDa on SDS-PAGE. This purification procedure resulted in 2.90-fold purification of lipase with a 24.10% final yield. The purified lipase presented maximal hydrolytic activity at a temperature of 55 ^°C, and pH of 7.0. Lipase activity was stimulated by Triton X-100 and SDS with Mg²⁺ and Ca²⁺ metals employ a positive effect and outlast its stable in organic solvent i.e. methanol and ethanol.

Keywords: 16s rRNA; Bacillus methylotrophicus Ps3; Characterization; Detergent; Lipase; Purification.