The family Theraphosidae is recorded from Greece for the first time with the description of a new species, Chaetopelma lymberakisi sp. n. Also, the male of Macrothele cretica Kulczyński, 1903 is re-described and the female of the same species is described for the first time.
Keywords: Araneae, Crete, mygalomorphs, new species, spiders, Taxonomy.