Circular dichroism (CD) of nanostructures is in great demand for applications in biological molecules, photocurrent devices, and photocatalysis. Planar nanostructures can be prepared in a concise manner, and their CD effects have gained much research interest. In this study, tilted zigzag-shaped nanohole (TZSN) arrays are proposed, and the CD effect is studied by the finite element method. A strong resonance occurs in the gap by tuning the charge distributions between adjacent nanoholes. Meanwhile, the CD effect of TZSN arrays is strongly dependent on the structural parameters of TZSN. Results provide a novel method for tuning the CD effects of nanohole arrays on a film.