Disruption of acdS gene reduces plant growth promotion activity and maize saline stress resistance by Rahnella aquatilis HX2

Abstract

Rahnella aquatilis HX2 was isolated from Beijing vineyard soil and used as a plant growth-promoting rhizobacterium in the field. Previous studies have shown that it has a broad in vitro antimicrobial spectrum and could inhibit a variety of plant pathogenic bacteria and fungi. In this study, a gene, acdS, encoding 1-aminocyclopropane-1-carboxylic acid-deaminase was disrupted by in-frame deletion in the HX2 strain. Compared to the wild-type, the acdS-mutant had higher rates of nitrogen fixation, reduced indole-3-acetic acid production, lowered efficacy as a biological control agent against the grape crown gall pathogen Agrobacterium vitis. Under saline stress conditions, plant height, above-ground fresh weight, root fresh weight of corn plants were increased by treatment with HX2 but this increase was compromised by the disruption of acdS gene. Our data confirmed the function of HX2 on plant growth promoting and demonstrated that acdS gene plays a major role in its PGPR activities.

Keywords: ACC deaminase gene; Rahnella aquatilis; plant growth promotion; salt stress.