We explore spatial control of the formation of core-satellite nanoassemblies on glass substrates. UV irradiation leads to the photooxidative desorption of thiol linkers from gold nanoparticles deposited on the substrates, thereby prohibiting further assembly in the irradiated region. The distribution of assemblies and monomers yields a pattern with stark contrasts in colour and Raman enhancement. Our findings can be utilised in the fabrication of microfluidic SERS sensors, colour displays, photonic devices, and metamaterials.