Abstract
Trichobamide A (1), a novel pyrrocidine alkaloid with an unprecedented tetrahydro-5H-furo[2,3-b]pyrrol-5-one moiety, was isolated from the ascidian-derived fungus Trichobotrys effuse 4729. Trichobamide A (1) showed significant inhibition of the proliferation of two glioma cell lines, U251 and SNB19. It induced the up-regulation of P53 expression, which in turn induces the up-regulation of downstream pro-apoptotic gene expression and the down-regulation of anti-apoptotic gene expression.
MeSH terms
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Animals
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Antineoplastic Agents / chemistry*
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology
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Brain Neoplasms / metabolism
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Brain Neoplasms / pathology
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Crystallography, X-Ray
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Down-Regulation / drug effects
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Fungi / chemistry*
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Fungi / isolation & purification
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Fungi / metabolism
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Glioma / metabolism
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Glioma / pathology
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Humans
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Magnetic Resonance Spectroscopy
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Molecular Conformation
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Proto-Oncogene Proteins c-bcl-2 / genetics
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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Pyrroles / chemistry*
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Pyrroles / isolation & purification
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Pyrroles / pharmacology
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism
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Up-Regulation / drug effects
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Urochordata / microbiology
Substances
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Antineoplastic Agents
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Proto-Oncogene Proteins c-bcl-2
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Pyrroles
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Tumor Suppressor Protein p53