A free-living soil amoeba Thecamoeba quadrilineata (Carter, 1856) Lepşi, 1960 (Amoebozoa: Thecamoebidae) was isolated from endozoic conditions for the first time. Presence of amoebae was detected after 4 days following inoculation of the gut of the earthworm Lumbricus terrestris on agar plate with Escherichia coli. On the basis of our isolation, we consider T. quadrilineata as further amphizoic amoeba species. This study enlarges the range of amphizoic tendency in members of the genus Thecamoeba and stresses the need for further research on the pathogenic potential of Thecamoeba species.
Keywords: Amphizoic tendency; Earthworm; Free-living amoeba; Gut; Thecamoebidae.