Aims: We aimed to assess the association of postpartum maternal serum concentration of orexin-A with postpartum marital satisfaction considering the effect of mode of delivery as an influential factor. Methods: This cohort study conducted among third trimester pregnant women, who met our eligibility criteria. Postpartum maternal and cord serum concentration of orexin-A were measured and their association with postpartum marital satisfaction were assessed considering the impact of mode of delivery. Results: There was a statistically significant positive association between postpartum maternal and cord serum levels of orexin-A (r = 0.79, p < 0.001) and postpartum marital satisfaction among women with cesarean section (r = 0.31, p < 0.01). The maternal orexin-A level of women delivered with cesarean section who had post-partum marital dissatisfaction was significantly lower than those one with marital satisfaction (84.13 ± 95.88 vs. 153.08 ± 95.88 pg/ml, p = 0.04). Logistic regression model showed that the type of delivery was not related to marital satisfaction (OR = 1.7, 95%CI: 0.6, 4.8), p = 0.280). Conclusions: The postpartum maternal serum orexin-A level was associated with marital satisfaction in women delivered through cesarean section.