The actin cytoskeleton is important for rotavirus internalization and RNA genome replication

Oscar Trejo-Cerro; Nayeli Aguilar-Hernández; Daniela Silva-Ayala; Susana López; Carlos F Arias

doi:10.1016/j.virusres.2019.01.003

The actin cytoskeleton is important for rotavirus internalization and RNA genome replication

Virus Res. 2019 Apr 2:263:27-33. doi: 10.1016/j.virusres.2019.01.003. Epub 2019 Jan 9.

Authors

Oscar Trejo-Cerro¹, Nayeli Aguilar-Hernández¹, Daniela Silva-Ayala¹, Susana López¹, Carlos F Arias²

Affiliations

¹ Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico.
² Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62210, Mexico. Electronic address: arias@ibt.unam.mx.

Abstract

Numerous host factors are required for the efficient replication of rotavirus, including the activation and inactivation of several cell signaling pathways. One of the cellular structures that are reorganized during rotavirus infection is the actin cytoskeleton. In this work, we report that the dynamics of the actin microfilaments are important at different stages of the virus life cycle, specifically, during virus internalization and viral RNA synthesis at 6 h post-infection. Our results show that the actin-binding proteins alpha-actinin 4 and Diaph, as well as the Rho-family small GTPase Cdc42 are necessary for an efficient virus entry, while GTPase Rac1 is required for maximal viral RNA synthesis.

Keywords: Actin cytoskeleton; Rotavirus; Rotavirus genome replication; Rotavirus internalization.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actin Cytoskeleton / metabolism*
Animals
Cell Line
Epithelial Cells / virology
Host-Pathogen Interactions*
Macaca mulatta
RNA, Viral / biosynthesis*
Rotavirus / physiology*
Virus Internalization*
Virus Replication*

Substances

RNA, Viral