Frequency of 16S ribosomal RNA methyltransferase detection among Escherichia coli and Klebsiella pneumoniae clinical isolates obtained from patients in Canadian hospitals (CANWARD, 2013-2017)

Andrew Walkty; James A Karlowsky; Melanie R Baxter; Heather J Adam; David Boyd; Amrita Bharat; Michael R Mulvey; Marthe Charles; Marco Bergevin; George G Zhanel

doi:10.1016/j.diagmicrobio.2018.12.005

Frequency of 16S ribosomal RNA methyltransferase detection among Escherichia coli and Klebsiella pneumoniae clinical isolates obtained from patients in Canadian hospitals (CANWARD, 2013-2017)

Diagn Microbiol Infect Dis. 2019 Jun;94(2):199-201. doi: 10.1016/j.diagmicrobio.2018.12.005. Epub 2018 Dec 14.

Authors

Affiliations

¹ Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada; Shared Health, Winnipeg, Canada. Electronic address: AWalkty@sharedhealthmb.ca.
² Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada; Shared Health, Winnipeg, Canada.
³ Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Canada.
⁴ Public Health Agency of Canada, National Microbiology Laboratory, Winnipeg, Canada.
⁵ Vancouver General Hospital, Vancouver, Canada.
⁶ Hôpital Cité-de-la-Santé, Laval, Quebec.

PMID: 30638654
DOI: 10.1016/j.diagmicrobio.2018.12.005

Abstract

Pan-aminoglycoside (amikacin, gentamicin, tobramycin, plazomicin)-resistant Escherichia coli and Klebsiella pneumoniae clinical isolates from patients in Canadian Hospitals (2013-2017) were evaluated by whole genome sequencing for 16S ribosomal RNA methyltransferase genes. The rmtB gene was detected in 2 isolates (1 of 3094 E. coli [0.03%], 1 of 1039 K. pneumoniae [0.1%]).

Keywords: 16S rRNA methyltransferase; Aminoglycoside; Antimicrobial resistance; Gram-negative; Plazomicin; rmtB.

MeSH terms

Aminoglycosides / pharmacology
Anti-Bacterial Agents / pharmacology
Canada / epidemiology
Drug Resistance, Multiple, Bacterial
Escherichia coli / enzymology*
Escherichia coli / genetics
Escherichia coli / isolation & purification
Escherichia coli Infections / epidemiology
Escherichia coli Infections / microbiology*
Hospitals
Humans
Klebsiella Infections / epidemiology
Klebsiella Infections / microbiology*
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / enzymology*
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / isolation & purification
Methyltransferases / genetics*
Methyltransferases / metabolism
RNA, Ribosomal, 16S / metabolism*
Sequence Analysis, DNA
Whole Genome Sequencing

Substances

Aminoglycosides
Anti-Bacterial Agents
RNA, Ribosomal, 16S
Methyltransferases
rRNA (adenosine-O-2'-)methyltransferase