Reverse Micelle Encapsulation of Proteins for NMR Spectroscopy

Methods Enzymol. 2019:615:43-75. doi: 10.1016/bs.mie.2018.08.032. Epub 2018 Dec 10.

Abstract

Reverse micelle (RM) encapsulation of proteins for NMR spectroscopy has many advantages over standard NMR methods such as enhanced tumbling and improved sensitivity. It has opened many otherwise difficult lines of investigation including the study of membrane-associated proteins, large soluble proteins, unstable protein states, and the study of protein surface hydration dynamics. Recent technological developments have extended the ability of RM encapsulation with high structural fidelity for nearly all proteins and thereby allow high-quality state-of-the-art NMR spectroscopy. Optimal conditions are achieved using a streamlined screening protocol, which is described here. Commonly studied proteins spanning a range of molecular weights are used as examples. Very low-viscosity alkane solvents, such as propane or ethane, are useful for studying very large proteins but require the use of specialized equipment to permit preparation and maintenance of well-behaved solutions under elevated pressure. The procedures for the preparation and use of solutions of RMs in liquefied ethane and propane are described. The focus of this chapter is to provide procedures to optimally encapsulate proteins in reverse micelles for modern NMR applications.

Keywords: Low-viscosity fluids; Molecular reorientation; NMR spectroscopy; Reverse micelle encapsulation; Structure determination; Surfactant systems.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bacteria / metabolism
  • Cytochromes c / chemistry
  • Flavodoxin / chemistry
  • Magnetic Resonance Spectroscopy / methods*
  • Membrane Proteins / chemistry
  • Micelles*
  • Molecular Weight
  • Proteins / chemistry*
  • Solvents

Substances

  • Flavodoxin
  • Membrane Proteins
  • Micelles
  • Proteins
  • Solvents
  • Cytochromes c