Objective: To observe the effect and mechanism of resveratrol (Res) on isoprotere- nol (ISO) induced cardiomyocyte apoptosis rats.
Methods: Primary cultured neonatal cardiomyocyte ap- optosis rat model was established using ISO. Apoptosis cells were then randomly divided into 4 groups, i. e., the normal control group (non-serum DMEM culture fluid) , the model group (non-serum DMEM culture fluid + ISO 1 μmol/L for 48 h) , the Res + ISO group (ISO 1 μmol/L + Res 50 μmoI/L for 48 h) , the Res control group. (non-serum DMEM culture fluid + Res 50 l_mol/L). The apoptosis rate was measured by Hochest33258 staining. Ultrastructural changes of cardiomyocyte were observed by electron microscope. Leakage of lactate dehydrogenase (LDH) in the culture fluid was measured. Protein expressions of BcI-2 and Bax were detected using Western blot. Results The count of cardiomyocytes were reduced and the nucleus shape was irregular. The apoptosis bodies were visible and the apoptosis rate was increased in the model group. The cell membrane was complete with clear nuclear membrane in the Res + ISO group and the Res control group. Nuclear chromatin was concentrated and cell injured degree was attenuated in the Res +ISO group and the Res control group. Compared with the normal control group, the apoptosis rate and LDH leakage increased, the protein expression of Bcl-2 was down-regulated, and the expression of Bax was up-regulated in the model group (P <0. 05, P <0. 01). Compared with the model group, the apoptosis rate and LDH leakage decreased, the protein expression of Bcl-2 was up-regulated, and the expression of Bax was down-regulated in the Res + ISO group and the Res control group (P <0. 05).
Conclusion: Res could obviously attenuate ISO induced cardiomyocyte apoptosis, and its mechanism might be associated with reversing protein expressions of Bcl-2 and Bax.