Genomic analysis of three Clostridioides difficile isolates from urban water sources

Daniela Numberger; Thomas Riedel; Gayle McEwen; Ulrich Nübel; Martinique Frentrup; Isabel Schober; Boyke Bunk; Cathrin Spröer; Jörg Overmann; Hans-Peter Grossart; Alex D Greenwood

doi:10.1016/j.anaerobe.2019.01.002

Genomic analysis of three Clostridioides difficile isolates from urban water sources

Anaerobe. 2019 Apr:56:22-26. doi: 10.1016/j.anaerobe.2019.01.002. Epub 2019 Jan 8.

Affiliations

¹ Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, 10315, Berlin, Germany.
² Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124, Braunschweig, Germany.
³ Leibniz Institute of Freshwater Ecology and Inland Fisheries, Zur alten Fischerhütte 2, 16775, Stechlin, Germany; University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Strasse 24-25, 14476, Potsdam, Germany. Electronic address: hgrossart@igb-berlin.de.
⁴ Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Strasse 17, 10315, Berlin, Germany; Freie Universität Berlin, Department of Veterinary Medicine, Institute for Virology, Robert-von-Ostertag-Strasse 7-13, 14163, Berlin, Germany. Electronic address: greenwood@izw-berlin.de.

PMID: 30633971
DOI: 10.1016/j.anaerobe.2019.01.002

Abstract

We investigated inflow of a wastewater treatment plant and sediment of an urban lake for the presence of Clostridioides difficile by cultivation and PCR. Among seven colonies we sequenced the complete genomes of three: two non-toxigenic isolates from wastewater and one toxigenic isolate from the urban lake. For all obtained isolates, a close genomic relationship with human-derived isolates was observed.

Keywords: Clostridioides difficile; Genomes; Sediment; Wastewater.

MeSH terms

Bacteriological Techniques
Berlin
Clostridioides difficile / classification
Clostridioides difficile / genetics*
Clostridioides difficile / isolation & purification*
Genome, Bacterial*
Genomics
Genotype
Phylogeny
Polymerase Chain Reaction
Sequence Analysis, DNA
Water Microbiology*