A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System

Barbora Lavickova; Sebastian J Maerkl

doi:10.1021/acssynbio.8b00427

A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System

ACS Synth Biol. 2019 Feb 15;8(2):455-462. doi: 10.1021/acssynbio.8b00427. Epub 2019 Jan 25.

Authors

Barbora Lavickova¹, Sebastian J Maerkl¹

Affiliation

¹ Institute of Bioengineering, School of Engineering , École Polytechnique Fédérale de Lausanne , Lausanne , 1015 , Switzerland.

PMID: 30632751
DOI: 10.1021/acssynbio.8b00427

Abstract

We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription-translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 μg/mL at a cost of 0.09 USD/μL, leading to a 14-fold improvement in cost normalized protein synthesis yield over existing PURE systems. The one-pot method makes the PURE system easy to generate and allows it to be readily optimized and modified.

Keywords: PURE system; cell-free transcription and translation; protein expression; synthetic biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell-Free System*
Synthetic Biology / methods*