Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses

Virol J. 2019 Jan 10;16(1):6. doi: 10.1186/s12985-018-1111-7.

Abstract

Background: Waterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important.

Results: A duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm = 87.3 ± 0.26 °C or Tm = 85.4 ± 0.23 °C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded.

Conclusion: This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections.

Keywords: Duplex real-time PCR; GPV; MDPV; Parvovirus; SYBR Green I.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzothiazoles
  • Cloaca / virology
  • Diamines
  • Ducks / virology*
  • Geese / virology*
  • Genome, Viral
  • Organic Chemicals
  • Oropharynx / virology
  • Parvoviridae Infections / diagnosis
  • Parvoviridae Infections / veterinary*
  • Parvovirus / classification*
  • Parvovirus / isolation & purification
  • Phylogeny
  • Poultry Diseases / diagnosis*
  • Poultry Diseases / virology
  • Quinolines
  • Real-Time Polymerase Chain Reaction / methods*
  • Transition Temperature
  • Viral Load

Substances

  • Benzothiazoles
  • Diamines
  • Organic Chemicals
  • Quinolines
  • SYBR Green I