Facile approach to prepare HSA-templated MnO2 nanosheets as oxidase mimic for colorimetric detection of glutathione

Jia Ge; Ren Cai; Xigao Chen; Qiong Wu; Liangliang Zhang; Ying Jiang; Cheng Cui; Shuo Wan; Weihong Tan

doi:10.1016/j.talanta.2018.11.024

Facile approach to prepare HSA-templated MnO₂ nanosheets as oxidase mimic for colorimetric detection of glutathione

Talanta. 2019 Apr 1:195:40-45. doi: 10.1016/j.talanta.2018.11.024. Epub 2018 Nov 10.

Authors

Jia Ge¹, Ren Cai², Xigao Chen², Qiong Wu², Liangliang Zhang³, Ying Jiang², Cheng Cui², Shuo Wan², Weihong Tan⁴

Affiliations

¹ College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China; Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, United States; State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, and College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. Electronic address: jiage0630@hnu.edu.cn.
² Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, United States.
³ Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, United States; State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
⁴ Center for Research at Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, United States; State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, and College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China. Electronic address: tan@chem.ufl.edu.

PMID: 30625560
DOI: 10.1016/j.talanta.2018.11.024

Abstract

In this work, a simple, rapid, and highly sensitive colorimetric assay for the determination of glutathione (GSH) was developed. It employs human serum albumin (HSA)-templated MnO₂ nanosheets as an artificial oxidase. HSA-templated MnO₂ nanosheets can oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to a blue oxTMB product with a significant increase in absorbance at 652 nm in the absence of H₂O₂. When GSH is introduced, the MnO₂ nanosheets are reduced to Mn²⁺ ions, thereby inhibiting the formation of oxTMB. Based on these findings, a simple colorimetric assay was developed for the detection GSH in the range of 10 nM to 5 μM with a low detection limit of 5.6 nM. Importantly, the proposed method was successfully used for quantitative determination of GSH in biological fluids, such as human serum samples.

Keywords: Colorimetric; Glutathione; HSA; MnO(2) nanosheets; Oxidase.

MeSH terms

Biomimetics
Colorimetry
Glutathione / blood*
Glutathione / chemistry
Manganese Compounds / chemistry*
Nanostructures / chemistry*
Oxides / chemistry*
Oxidoreductases / chemistry
Serum Albumin, Human / chemistry*

Substances

Manganese Compounds
Oxides
manganese oxide
Oxidoreductases
Glutathione
Serum Albumin, Human