Saccharomyces-derived carbon dots for biosensing pH and vitamin B 12

Ying Yu; Chunguang Li; Cailing Chen; He Huang; Chen Liang; Yue Lou; Xiao-Bo Chen; Zhan Shi; Shouhua Feng

doi:10.1016/j.talanta.2018.11.010

Saccharomyces-derived carbon dots for biosensing pH and vitamin B 12

Talanta. 2019 Apr 1:195:117-126. doi: 10.1016/j.talanta.2018.11.010. Epub 2018 Nov 5.

Authors

Ying Yu¹, Chunguang Li², Cailing Chen¹, He Huang¹, Chen Liang¹, Yue Lou¹, Xiao-Bo Chen³, Zhan Shi⁴, Shouhua Feng¹

Affiliations

¹ State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun 130012, People's Republic of China.
² State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun 130012, People's Republic of China. Electronic address: cglee@jlu.edu.cn.
³ School of Engineering, RMIT University, Carlton, VIC 3053, Australia.
⁴ State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun 130012, People's Republic of China. Electronic address: zshi@mail.jlu.edu.cn.

PMID: 30625521
DOI: 10.1016/j.talanta.2018.11.010

Abstract

Photoluminescence(PL) nano-biosensors that can be used for accurately and reliably monitoring pH and vitamin hold a great promise in biology and medicine. Herein, a high quantum yield of 16% saccharomyces-derived N-doped carbon dots (s-N-CDs) was synthesized through a simple and one-pot microwave-assisted hydrothermal approach. The produced s-N-CDs are an excellent multi-functional biosensor for the applications of pH sensing and vitamin probing. Fluorescence intensity and fluorescence lifetime dramatically increases with pH decreasing from 14 to 2. Moreover, the fluorescence intensity presents highly reversible abilty from 13 to 2 without any profound attenuation after ten consecutive circles. More importantly, the CDs prepared herein are sound option for assaying cobalamin (VB 12) based fluorescence resonance energy transfer (FRET) with a superior low detection limit of 2.19 μM.

Keywords: Biosensor; Carbon dots; Saccharomyces; Vitamin B 12; pH.

MeSH terms

Biosensing Techniques*
Carbon / chemistry
Ethylenediamines
Fluorescence Resonance Energy Transfer
Hydrogen-Ion Concentration*
Limit of Detection
Nanoparticles / chemistry
Nitrogen / chemistry
Saccharomyces
Vitamin B 12 / analysis*
Vitamin B 12 / chemistry
Vitamins

Substances

Ethylenediamines
Vitamins
ethylenediamine
Carbon
Nitrogen
Vitamin B 12