Generation of induced pluripotent stem cell line (ZZUi0013-A) from a 65-year-old patient with a novel MEOX2 gene mutation in Alzheimer's disease

Stem Cell Res. 2019 Jan:34:101366. doi: 10.1016/j.scr.2018.101366. Epub 2018 Dec 27.

Abstract

MEOX2 mutation has been reported as a potential cause of familial Alzheimer's disease. Recently, a novel MEOX2 mutation was identified in a family with Alzheimer's disease. The dermal fibroblasts of the patient were obtained and successfully transformed into induced pluripotent stem cells (iPSCs), employing episomal plasmids expressing OCT3/4, SOX2, KLF4, LIN28, and L-MYC. Our model may offer a good platform for further research on the pathomechanism, drug testing, and gene therapy of this disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Alzheimer Disease / genetics*
  • Alzheimer Disease / pathology*
  • Animals
  • Base Sequence
  • Cell Culture Techniques / methods*
  • Cell Line
  • Homeodomain Proteins / genetics*
  • Humans
  • Induced Pluripotent Stem Cells / pathology*
  • Kruppel-Like Factor 4
  • Male
  • Mice, SCID
  • Mutation / genetics*

Substances

  • Homeodomain Proteins
  • KLF4 protein, human
  • Klf4 protein, mouse
  • Kruppel-Like Factor 4
  • MEOX2 protein, human