An inorganic magnetic fluorescent nanoprobe with favorable biocompatibility for dual-modality bioimaging and drug delivery

Hongen Guo; Yixia Zhang; Wenting Liang; Feifei Tai; Qingchen Dong; Ruiping Zhang; Baofeng Yu; Wai-Yeung Wong

doi:10.1016/j.jinorgbio.2018.12.002

An inorganic magnetic fluorescent nanoprobe with favorable biocompatibility for dual-modality bioimaging and drug delivery

J Inorg Biochem. 2019 Mar:192:72-81. doi: 10.1016/j.jinorgbio.2018.12.002. Epub 2018 Dec 10.

Authors

Hongen Guo¹, Yixia Zhang¹, Wenting Liang², Feifei Tai¹, Qingchen Dong³, Ruiping Zhang⁴, Baofeng Yu⁵, Wai-Yeung Wong⁶

Affiliations

¹ MOE Key Laboratory of Interface Science and Engineering in Advanced Materials, Research Center of Advanced Materials Science and Technology, Taiyuan University of Technology, No. 79 Yingze West Street, Taiyuan 030024, China.
² Institute of Environmental Sciences and Department of Chemistry, Shanxi University, Taiyuan 030006, China.
³ MOE Key Laboratory of Interface Science and Engineering in Advanced Materials, Research Center of Advanced Materials Science and Technology, Taiyuan University of Technology, No. 79 Yingze West Street, Taiyuan 030024, China. Electronic address: dongqingchen@tyut.edu.cn.
⁴ Imaging Department of Affiliated Tumor Hospital of Shanxi Medical University, Imaging Department of Shanxi Provincial Cancer Hospital, Taiyuan 030001, China. Electronic address: zrp_7142@sxmu.edu.cn.
⁵ Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China.
⁶ MOE Key Laboratory of Interface Science and Engineering in Advanced Materials, Research Center of Advanced Materials Science and Technology, Taiyuan University of Technology, No. 79 Yingze West Street, Taiyuan 030024, China. Electronic address: wai-yeung.wong@polyu.edu.hk.

PMID: 30612028
DOI: 10.1016/j.jinorgbio.2018.12.002

Abstract

In this work, we present the development of a high water soluble biological nanoprobe through the covalent bonding of β-CD onto the surface of iron oxide‑gold nanoclusters (Fe₃O₄@Au@β-CD). Fe₃O₄@Au@SiO₂ NPs were also prepared for comparison. The maximum emission peak of magnetic fluorescent NPs red-shifted by 30 nm and the lifetime was also elongated to 5.21 μs after surface modified with β-CD. The relaxivities and in vitro magnetic resonance imaging ability of the resultant magnetic fluorescent NPs were also studied, indicating that Fe₃O₄@Au@β-CD NPs have the lowest r₂/r₁ ratio and could be a potential T₂ contrast agent for MR imaging. The MTT assay proved that, Fe₃O₄@Au@β-CD NPs are of excellent water solubility and biocompatibility. In vitro confocal fluorescence imaging was also performed, manifesting that Fe₃O₄@Au@β-CD NPs can be selectively uptaken by gastric cancer cells (MGC-803) and exhibit red fluorescence in the cells. The preliminary drug loading and releasing measurements demonstrate that it can also act as targeted drug delivery nanosystem. All these experimental results indicate that, Fe₃O₄@Au@β-CD NPs hold great application perspective in the diagnosis and therapy of gastric cancer cells as biological nanoprobes.

Keywords: Biocompatibility; Drug delivery; Dual-modality imaging; Magnetic fluorescent nanocomposites; Multifunctional.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Contrast Media* / chemistry
Contrast Media* / pharmacology
Doxorubicin* / chemistry
Doxorubicin* / pharmacology
Drug Delivery Systems*
Gold* / chemistry
Gold* / pharmacology
Humans
Magnetic Resonance Imaging*
Magnetite Nanoparticles* / chemistry
Magnetite Nanoparticles* / therapeutic use
Stomach Neoplasms* / diagnostic imaging
Stomach Neoplasms* / drug therapy
Stomach Neoplasms* / metabolism

Substances

Contrast Media
Magnetite Nanoparticles
Gold
Doxorubicin