Analysis of Autophagy for Liver Pathogenesis

Methods Mol Biol. 2019:1880:481-489. doi: 10.1007/978-1-4939-8873-0_30.

Abstract

The autophagy pathway in hepatocytes is well characterized. Autophagy plays a critical role in the normal function of the liver. A growing number of studies suggest that there is a mechanistic relationship between autophagy and the pathogenesis of human diseases including liver diseases. Here we focus on the methods assessing the level of lipids, lipid peroxidation, and lipophagy in the liver, which would be particularly relevant to the study of fatty liver diseases.

Keywords: Autophagy; Fatty liver diseases; Hepatocytes; Immunofluorescence; Lipid droplets; Lipophagy; Reactive oxygen species.

MeSH terms

  • Animals
  • Autophagy / physiology*
  • Biological Assay / instrumentation
  • Biological Assay / methods*
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods
  • Cell Line
  • Fatty Liver / pathology
  • Hepatocytes / metabolism
  • Lipid Droplets / metabolism
  • Lipid Metabolism / physiology*
  • Lipids / analysis
  • Liver / metabolism*
  • Liver / pathology
  • Mice
  • Microscopy, Confocal / instrumentation
  • Microscopy, Confocal / methods
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods
  • Oxidative Stress / physiology
  • Reactive Oxygen Species / metabolism

Substances

  • Lipids
  • Reactive Oxygen Species