Genome Editing in Citrus Tree with CRISPR/Cas9

Hongge Jia; Xiuping Zou; Vladimir Orbovic; Nian Wang

doi:10.1007/978-1-4939-8991-1_17

Genome Editing in Citrus Tree with CRISPR/Cas9

Methods Mol Biol. 2019:1917:235-241. doi: 10.1007/978-1-4939-8991-1_17.

Authors

Hongge Jia¹, Xiuping Zou¹, Vladimir Orbovic², Nian Wang³

Affiliations

¹ Citrus Research and Education Center, Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences (IFAS), University of Florida, Lake Alfred, FL, USA.
² Citrus Research and Education Center, Institute of Food and Agricultural Sciences (IFAS), University of Florida, Lake Alfred, FL, USA.
³ Citrus Research and Education Center, Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences (IFAS), University of Florida, Lake Alfred, FL, USA. nianwang@ufl.edu.

PMID: 30610640
DOI: 10.1007/978-1-4939-8991-1_17

Abstract

CRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in transgenic citrus by Cas9/sgRNA, a two-component system derived from CRISPR-Cas9. Transgenic citrus plants can be created by Agrobacterium-mediated epicotyl transformation.

Keywords: Agrobacterium-mediated epicotyl transformation; Cas9/sgRNA; Citrus; CsLOB1; Genome editing.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Agrobacterium / genetics
CRISPR-Cas Systems / genetics*
Citrus / genetics*
Gene Editing / methods*
Plant Proteins / genetics*

Substances

Plant Proteins