CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation

Aimee Malzahn; Yong Zhang; Yiping Qi

doi:10.1007/978-1-4939-8991-1_7

CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation

Methods Mol Biol. 2019:1917:83-93. doi: 10.1007/978-1-4939-8991-1_7.

Authors

Aimee Malzahn¹, Yong Zhang², Yiping Qi^{3

4}

Affiliations

¹ Department of Plant Science and Landscape Architecture, University of Maryland College Park, College Park, MD, USA.
² Department of Biotechnology, School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.
³ Department of Plant Science and Landscape Architecture, University of Maryland College Park, College Park, MD, USA. yiping@umd.edu.
⁴ Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD, USA. yiping@umd.edu.

PMID: 30610630
DOI: 10.1007/978-1-4939-8991-1_7

Abstract

CRISPR systems have greatly promoted research in genome editing and transcriptional regulation. CRISPR-based transcriptional repression and activation systems will be valuable for applications in engineering plant immunity, boosting metabolic production, and enhancing our knowledge of gene regulatory networks. Multiplexing of CRISPR allows multiple genes to be targeted without significant additional effort. Here, we describe our CRISPR-Act2.0 system which is an improved multiplexing transcriptional activation system in plants.

Keywords: Artificial transcription factor; CRISPR; Gateway cloning; Golden Gate assembly; MCP-VP64; Multiplex; Plant transcriptional activation; dCas9-VP64.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

CRISPR-Cas Systems / genetics*
Clustered Regularly Interspaced Short Palindromic Repeats / genetics
Gene Editing / methods*
Gene Expression Regulation, Plant / genetics
Plant Immunity / genetics
Plant Immunity / physiology
Transcriptional Activation / genetics
Transcriptional Activation / physiology