Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis

Methods Mol Biol. 2019:1930:51-57. doi: 10.1007/978-1-4939-9036-8_7.

Abstract

The ability of activated T-lymphocytes to transmigrate toward certain chemokine is one of their characteristic functional properties. Here, we provide step-wise details about an in vitro technique to quantify the kinetics of chemotactic behavior of LFA-1-stimulated T-lymphocytes. The method described herein utilizes a noninvasive electrical impedance-based detection system to monitor T-cell chemotaxis in real-time.

Keywords: Chemoattractant; Chemotaxis; ICAM-1; LFA-1; SDF-1α; Transwell migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Tracking / methods*
  • Cells, Cultured
  • Chemotaxis*
  • Cytokines / metabolism*
  • Electric Impedance
  • Humans
  • Intercellular Adhesion Molecule-1 / metabolism
  • Lymphocyte Activation*
  • Lymphocyte Function-Associated Antigen-1 / metabolism*
  • Signal Transduction
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology
  • T-Lymphocytes / physiology*

Substances

  • Cytokines
  • ICAM1 protein, human
  • Lymphocyte Function-Associated Antigen-1
  • Intercellular Adhesion Molecule-1