A salting-out assisted liquid-liquid microextraction procedure for determination of cysteine followed by spectrophotometric detection

Alina Diuzheva; József Balogh; Yaroslav Studenyak; Zoltán Cziáky; József Jekő

doi:10.1016/j.talanta.2018.10.026

A salting-out assisted liquid-liquid microextraction procedure for determination of cysteine followed by spectrophotometric detection

Talanta. 2019 Mar 1:194:446-451. doi: 10.1016/j.talanta.2018.10.026. Epub 2018 Oct 14.

Authors

Alina Diuzheva¹, József Balogh², Yaroslav Studenyak³, Zoltán Cziáky⁴, József Jekő⁵

Affiliations

¹ Department of Analytical Chemistry, Pavol Jozef Šafárik University in Košice, Košice, Slovakia. Electronic address: alina.diuzheva@student.upjs.sk.
² Department of Chemistry, University of Nyíregyháza, Nyíregyháza, Hungary; Department of Analytical Chemistry, Uzhgorod National University, Uzhgorod, Ukraine.
³ Department of Analytical Chemistry, Uzhgorod National University, Uzhgorod, Ukraine.
⁴ Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, Hungary.
⁵ Department of Chemistry, University of Nyíregyháza, Nyíregyháza, Hungary; Agricultural and Molecular Research and Service Institute, University of Nyíregyháza, Nyíregyháza, Hungary.

PMID: 30609556
DOI: 10.1016/j.talanta.2018.10.026

Abstract

A new analytical method for sensitive determination of cysteine based on its interaction with phenazine methosulfate was developed using salting-out liquid-liquid microextraction followed by spectrophotometric detection. The mechanism of the reaction was studied and confirmed by Fourier transform infrared and mass spectroscopy. Experimental parameters affecting the extraction efficiency were investigated and under the optimal conditions, good linearity was observed in the range 0.2 - 6.0 µg mL^-1 with a correlation coefficient of 0.9972. The limit of detection and limit of quantification were found to be 0.07 and 0.21 µg mL ^-1, respectively. The enrichment factor was 25. The developed methodology was applied for analysis of cysteine in food supplements. The obtained data were in good agreement with LC-MS/MS analysis.

Keywords: Cysteine; Phenazine methosulfate; Salting-out assisted liquid-liquid microextraction; Spectrophotometry.

MeSH terms

Cysteine / analysis*
Cysteine / chemistry
Cysteine / isolation & purification*
Food Analysis
Liquid Phase Microextraction / methods*
Methylphenazonium Methosulfate / chemistry
Salts / chemistry*
Spectrophotometry*

Substances

Salts
Methylphenazonium Methosulfate
Cysteine