Luminescent silica mesoparticles for protein transduction

Yuri A Vorotnikov; Tatiana N Pozmogova; Anastasiya O Solovieva; Svetlana M Miroshnichenko; Elena V Vorontsova; Lidiya V Shestopalova; Yuri V Mironov; Michael A Shestopalov; Olga A Efremova

doi:10.1016/j.msec.2018.11.056

Luminescent silica mesoparticles for protein transduction

Mater Sci Eng C Mater Biol Appl. 2019 Mar:96:530-538. doi: 10.1016/j.msec.2018.11.056. Epub 2018 Nov 30.

Authors

Yuri A Vorotnikov¹, Tatiana N Pozmogova², Anastasiya O Solovieva³, Svetlana M Miroshnichenko³, Elena V Vorontsova³, Lidiya V Shestopalova⁴, Yuri V Mironov⁵, Michael A Shestopalov⁶, Olga A Efremova⁷

Affiliations

¹ Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev Ave., 630090 Novosibirsk, Russian Federation; Scientific Institute of Clinical and Experimental Lymphology - branch of ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russian Federation.
² Scientific Institute of Clinical and Experimental Lymphology - branch of ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russian Federation; Novosibirsk State University, 2 Pirogova str., 630090 Novosibirsk, Russian Federation.
³ Scientific Institute of Clinical and Experimental Lymphology - branch of ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russian Federation; Federal Research Center of Fundamental and Translational Medicine, 2 Timakova str., 630117 Novosibirsk, Russian Federation.
⁴ Novosibirsk State University, 2 Pirogova str., 630090 Novosibirsk, Russian Federation.
⁵ Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev Ave., 630090 Novosibirsk, Russian Federation; Novosibirsk State University, 2 Pirogova str., 630090 Novosibirsk, Russian Federation.
⁶ Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentiev Ave., 630090 Novosibirsk, Russian Federation; Scientific Institute of Clinical and Experimental Lymphology - branch of ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russian Federation; Novosibirsk State University, 2 Pirogova str., 630090 Novosibirsk, Russian Federation; Federal Research Center of Fundamental and Translational Medicine, 2 Timakova str., 630117 Novosibirsk, Russian Federation. Electronic address: shtopy@niic.nsc.ru.
⁷ Scientific Institute of Clinical and Experimental Lymphology - branch of ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russian Federation; Federal Research Center of Fundamental and Translational Medicine, 2 Timakova str., 630117 Novosibirsk, Russian Federation; School of Mathematics and Physical Sciences, University of Hull, Cottingham Road, HU6 7RX, Hull, UK. Electronic address: o.efremova@hull.ac.uk.

PMID: 30606563
DOI: 10.1016/j.msec.2018.11.056

Abstract

Unlike silica nanoparticles, the potential of silica mesoparticles (SMPs) (i.e. particles of submicron size) for biological applications in particular the in vitro (let alone in vivo) cellular delivery of biological cargo has so far not been sufficiently studied. Here we examine the potential of luminescent (namely, octahedral molybdenum cluster doped) SMPs synthesised by a simple one-pot reaction for the labelling of cells and for protein transduction into larynx carcinoma (Hep-2) cells using GFP as a model protein. Our data demonstrates that the SMPs internalise into the cells within half an hour. This results in cells that detectably luminesce via conventional methods. In addition, the particles are non-toxic both in darkness and upon photo-irradiation. The SMPs were modified to allow their functionalisation by a protein, which then delivered the protein (GFP) efficiently into the cells. Thus, the luminescent SMPs offer a cheap and trackable alternative to existing materials for cellular internalisation of proteins, such as the HIV TAT protein and commercial protein delivery agents (e.g. Pierce™).

Keywords: Cellular uptake; Cytotoxicity; Octahedral molybdenum cluster; Protein transduction; Silica.

MeSH terms

Cell Line, Tumor
Green Fluorescent Proteins* / chemistry
Green Fluorescent Proteins* / pharmacology
Humans
Luminescence*
Nanoparticles / chemistry*
Silicon Dioxide* / chemistry
Silicon Dioxide* / pharmacology

Substances

Green Fluorescent Proteins
Silicon Dioxide