Di(n-butyl) phthalate exposure impairs meiotic competence and development of mouse oocyte

Fei-Ping Li; Jie-Long Zhou; Ai-Wei Guo; Yu Liu; Fei Zhang; Bai-Hui Xu; Rui Liu; Ya-Long Wang; Ming-Huang Chen; Yan-Hong Lin; Shu-Wen He; Bao-Qiong Liao; Xian-Pei Fu; Hai-Long Wang

doi:10.1016/j.envpol.2018.12.077

Di(n-butyl) phthalate exposure impairs meiotic competence and development of mouse oocyte

Environ Pollut. 2019 Mar:246:597-607. doi: 10.1016/j.envpol.2018.12.077. Epub 2018 Dec 26.

Authors

Fei-Ping Li¹, Jie-Long Zhou², Ai-Wei Guo², Yu Liu³, Fei Zhang⁴, Bai-Hui Xu⁵, Rui Liu⁴, Ya-Long Wang⁵, Ming-Huang Chen⁴, Yan-Hong Lin⁶, Shu-Wen He⁵, Bao-Qiong Liao⁷, Xian-Pei Fu⁵, Hai-Long Wang⁸

Affiliations

¹ Organ Transplantation Institute, Medical College, Xiamen University, Xiamen, Fujian, China; College of Life Sciences, Southwest Forestry University, Kunming, Yunnan, China; Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen, Fujian, China; Chengdu Research Base of Giant Panda Breeding, Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu, Sichuan, China.
² College of Life Sciences, Southwest Forestry University, Kunming, Yunnan, China.
³ Organ Transplantation Institute, Medical College, Xiamen University, Xiamen, Fujian, China; Medical College, Guangxi University, Nanning, Guangxi, China.
⁴ Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen, Fujian, China; Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen, Fujian, China.
⁵ Organ Transplantation Institute, Medical College, Xiamen University, Xiamen, Fujian, China; Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen, Fujian, China.
⁶ Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen, Fujian, China; Department of Gynecology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
⁷ Organ Transplantation Institute, Medical College, Xiamen University, Xiamen, Fujian, China; Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen, Fujian, China; Department of Gynaecology and Obstetrics, Dongfang Affiliated Hospital of Xiamen University, Fuzhou, Fujian, China.
⁸ Organ Transplantation Institute, Medical College, Xiamen University, Xiamen, Fujian, China; Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen, Fujian, China. Electronic address: hailongwang@xmu.edu.cn.

PMID: 30605815
DOI: 10.1016/j.envpol.2018.12.077

Abstract

Di(n-butyl) phthalate (DBP) is extensively used in industrial applications as plasticizer and stabilizer and its presence in the environment may present health risks for human. Previous studies have demonstrated its mutagenic, teratogenic, and carcinogenic ability. However, its effect on mammalian oocyte maturation remains unknown. In this study, we examined the effect of DBP on oocyte maturation both in vitro and in vivo. Our results showed that DBP could significantly reduce mice oocyte germinal vesicle breakdown (GVBD) and polar body extrusion (PBE) rates. In addition, oocyte cytoskeleton was damaged and cortical granule-free domains (CGFDs) were also disrupted. Finally, DBP induced early apoptosis of oocyte and granulosa cells (GCs). Collectively, these data demonstrate that DBP could reduce meiosis competence and mouse oocyte development.

Keywords: Cytoskeletons; Dibutyl phthalate; Meiosis; Mice oocyte; Spindle.

MeSH terms

Animals
Apoptosis / drug effects*
Dibutyl Phthalate / toxicity*
Environmental Pollutants / toxicity*
Female
Granulosa Cells / drug effects
Granulosa Cells / pathology
Humans
Meiosis / drug effects*
Mice
Mice, Inbred ICR
Oocytes / drug effects*
Oocytes / growth & development
Oocytes / pathology

Substances

Environmental Pollutants
Dibutyl Phthalate