Micromonospora craniellae sp. nov., isolated from a marine sponge, and reclassification of Jishengella endophytica as Micromonospora endophytica comb. nov

Abstract

A novel marine actinomycete, designated LHW63014^T, isolated from a marine sponge, Craniella species, collected in the South China Sea, was examined using a polyphasic approach. The 16S rRNA gene sequence of strain LHW63014^T showed highest similarities to Jishengella endophytica 202201^T (98.9 %), Micromonospora olivasterospora DSM 43868^T (98.7 %), Micromonospora maris AB-18-032^T (98.6 %) and Micromonospora gifhornensis DSM 44337^T (98.6 %). Phylogenetic analyses of the family Micromonosporaceae based on the 16S rRNA and gyrB gene sequences indicated that strain LHW63014^T and J. endophytica 202201^T located within the genus Micromonospora. Morphological and chemotaxonomic characteristics confirmed their affiliation to the genus Micromonospora. Based on phenotypic characteristics, phylogenetic data and digital DNA-DNA hybridization results, strain LHW63014^T could be distinguished from its closest taxa, representing a novel species of the genus Micromonospora, for which the name Micromonospora craniellae sp. nov., is proposed, with the type strain LHW63014^T (=DSM 106193^T=CCTCC AA 2018012^T). It is also proposed that J. endophytica be transferred to genus Micromonospora as Micromonospora endophytica comb. nov. (type strain 202201^T =CGMCC 4.5597^T=DSM 45430^T).

Keywords: Micromonospora craniellae sp. nov.; Micromonospora endophytica comb. nov.; marine sponge.