Amycolatopsis panacis sp. nov., isolated from Panax notoginseng rhizospheric soil

Abstract

A novel Gram-positive bacterium, designated strain YIM PH21725^T, was isolated from a sample of rhizospheric soil of Panaxnotoginseng cultivated in Anning, Yunnan. The strain contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The main fatty acids identified were C17 : 0, iso-C15 : 0, iso-C16 : 0 and C16 : 0. The main menaquinone was MK-9 (H4). The polar lipids included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phospholipids and phospholipids of an unidentified structure containing glucosamine. The G+C content of genomic DNA was 69.43 mol%. On the basis of its 16S rRNA gene sequence, strain YIM PH21725^T should belong to the genus Amycolatopsis, and was closely related to Amycolatopsis sulphurea DSM 46092^T (98.57 %), Amycolatopsis jejuensis JCM13280^T (97.27 %), Amycolatopsis jiangsuensis KCTC 19885^T (96.88 %) and Amycolatopsis ultiminotia JCM 16989^T (96.8 %). The phenotypic, chemotaxonomic, phylogenetic and digital DNA-DNA hybridization results clearly indicated that strain YIM PH21725^T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsispanacis sp. nov. is proposed. The type strain is YIM PH21725^T (=CCTCC AA 2017044^T=KCTC 49031^T=DSM 105902^T).

Keywords: Actinomycetes; Amycolatopsis panacis sp. nov; Panax notoginseng; rhizospheric soil.